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Research Associate CV
Dr
.
Elena
Vasquez
Research
Associate
·
Molecular
Biology
elena
.
vasquez
@
researchlab
.
org
+1 (415) 555-0192
San
Francisco
,
CA
linkedin
.
com
/
in
/
elenavasquez
github
.
com
/
e
-
venasquez
PROFESSIONAL
SUMMARY
Research
Associate
with
5+
years
of
experience
in
molecular
biology
,
genomic
analysis
,
and
cell
-
based
assays
.
Skilled
in
CRISPR
-
Cas
9
gene
editing
,
high
-
throughput
screening
,
and
bioinformatics
pipeline
development
.
Proven
track
record
of
contributing
to
peer
-
reviewed
publications
and
managing
cross
-
functional
research
projects
in
academic
and
biotech
settings
.
Passionate
about
translating
fundamental
biological
discoveries
into
therapeutic
applications
.
RESEARCH
EXPERIENCE
Research
Associate
Sep
2022 –
Present
Genentech
Inc
. ·
South
San
Francisco
,
CA
Research
Associate
Jan
2020 –
Aug
2022
Stanford
University
School
of
Medicine
·
Stanford
,
CA
Lead
a
team
of
3
research
assistants
investigating
epigenetic
regulation
in
oncology
targets
using
CRISPR
-
dCas
9
fusion
systems
.
Designed
and
executed
pooled
CRISPR
screens
(
genome
-
scale
and
targeted
)
identifying
14
novel
synthetic
lethal
interactions
in
triple
-
negative
breast
cancer
cell
lines
.
Developed
an
automated
high
-
content
imaging
pipeline
reducing
data
acquisition
time
by
40%
while
maintaining
>95%
assay
reproducibility
.
Collaborated
with
computational
biology
group
to
integrate
single
-
cell
RNA
-
seq
data
with
CRISPR
screen
results
,
uncovering
two
previously
unknown
resistance
mechanisms
.
Presented
findings
at
3
international
conferences
including
AACR
Annual
Meeting
2024
and
Keystone
Symposia
.
Managed
daily
operations
of
a
molecular
biology
lab
studying
RNA
-
binding
protein
dysregulation
in
neurodegeneration
.
Executed
RNA
-
seq
,
RIP
-
seq
,
and
CLIP
-
seq
library
preparation
protocols
;
analyzed
differential
expression
and
splicing
events
using
STAR
,
DESeq
2,
and
rMATS
.
Optimized
lentiviral
production
and
transduction
protocols
achieving
titers
consistently
above
1×10
⁸
TU
/
mL
for
primary
neuron
and
iPSC
-
derived
models
.
Trained
and
mentored
6
graduate
students
and
4
undergraduate
researchers
in
molecular
cloning
,
cell
culture
,
and
data
analysis
best
practices
.
Co
-
authored
3
manuscripts
in
high
-
impact
journals
(
Nature
Neuroscience
,
Molecular
Cell
)
with
one
first
-
author
publication
.
1 / 3
Junior
Research
Scientist
Jun
2017 –
Dec
2019
Broad
Institute
of
MIT
and
Harvard
·
Cambridge
,
MA
EDUCAT ION
Ph
.
D
.
in
Molecular
and
Cell
Biology
2012 – 2017
University
of
California
,
Berkeley
GPA
: 3.92
Dissertation
: “
Mechanisms
of
RNA
localization
in
neuronal
development
and
plasticity
.”
Advisor
:
Dr
.
Jennifer
A
.
Doudna
.
NIH
NRSA
Predoctoral
Fellow
.
B
.
S
.
in
Biochemistry
and
Molecular
Biology
2008 – 2012
University
of
Michigan
,
Ann
Arbor
GPA
: 3.88 ·
Magna
Cum
Laude
Honors
thesis
: “
Characterization
of
SUMOylation
dynamics
in
DNA
damage
repair
pathways
.”
Undergraduate
Research
Opportunity
Program
grant
recipient
.
T ECHNICAL
SKILLS
Molecular
CRISPR
-
Cas
9 (
pooled
,
arrayed
,
prime
editing
),
qPCR
/
ddPCR
,
cloning
,
Gibson
assembly
,
Western
blot
,
Co
-
IP
,
RIP
-
seq
,
CLIP
-
seq
Cell
Biology
Mammalian
cell
culture
(
HEK
293
T
,
HeLa
,
K
562,
iPSC
-
derived
neurons
),
lentivirus
/
AAV
production
,
flow
cytometry
,
high
-
content
imaging
(
Opera
Phenix
)
Genomics
RNA
-
seq
,
single
-
cell
RNA
-
seq
(10
x
Genomics
),
ATAC
-
seq
,
ChIP
-
seq
,
whole
-
exome
sequencing
,
CRISPR
amplicon
sequencing
Bioinformatics
Python
(
pandas
,
NumPy
,
Scanpy
),
R
(
DESeq
2,
Seurat
,
ggplot
2),
Snakemake
,
bash
,
Git
,
Docker
,
SLURM
HPC
,
AWS
cloud
computing
Assays
Cell
viability
(
CellTiter
-
Glo
,
MTT
),
apoptosis
(
Annexin
-
V
,
caspase
activity
),
proliferation
(
EdU
,
CFSE
),
ELISA
,
AlphaLISA
,
IncuCyte
live
-
cell
analysis
Other
ELN
(
Benchling
,
LabArchives
),
GraphPad
Prism
,
ImageJ
/
Fiji
,
FlowJo
,
Adobe
Illustrator
,
project
management
(
Asana
,
Trello
)
SELECT ED
PUBLICAT IONS
Vasquez
E
.
,
Park
J
.,
Liu
A
.
H
.,
et
al
. (2024).
Epigenetic
rewiring
drives
resistance
to
CDK
4/6
inhibitors
in
triple
-
negative
breast
cancer
.
Nature
Cell
Biology
,
26(4), 612–
625.
Nguyen
T
.
Q
.,
Vasquez
E
.
,
Soriano
A
.,
et
al
. (2023).
A
genome
-
wide
CRISPR
screen
identifies
novel
regulators
of
tau
aggregation
in
iPSC
-
derived
neurons
.
Neuron
,
111(8), 1201–1218.
Vasquez
E
.
,
Chen
Y
.,
O
'
Malley
M
. (2022).
RNA
-
binding
protein
FMRP
controls
local
translation
of
synaptic
plasticity
genes
in
hippocampal
neurons
.
Molecular
Cell
,
82(3), 543–560.
Schmidt
K
.,
Vasquez
E
.
,
Doudna
J
.
A
. (2021).
Kinetic
determinants
of
CRISPR
-
Cas
9
off
-
target
activity
in
human
cells
.
Nature
Communications
,
12, 3154.
HONORS
&
AWARDS
Contributed
to
a
large
-
scale
functional
genomics
project
characterizing
essential
genes
across
350+
cancer
cell
lines
using
CRISPR
-
Cas
9
dropout
screens
.
Developed
and
validated
a
multiplexed
qPCR
-
based
genotyping
panel
for
isogenic
cell
line
authentication
,
achieving
99.8%
concordance
with
WGS
data
.
Processed
and
analyzed
NGS
data
(
whole
-
exome
,
targeted
amplicon
,
CRISPR
amplicon
)
using
a
custom
Snakemake
pipeline
deployed
on
an
HPC
cluster
.
Maintained
laboratory
inventory
,
equipment
calibration
schedules
,
and
biosafety
compliance
documentation
across
3
shared
lab
spaces
.
NIH
Ruth
L
.
Kirschstein
NRSA
Predoctoral
Fellowship
(
F
31) · 2015–2017
Keystone
Symposia
Future
of
Science
Scholarship
· 2024
Genentech
Postdoctoral
Publication
Award
·
2023
UC
Berkeley
Graduate
Research
Fellowship
·
2013–2017
Phi
Beta
Kappa
·
University
of
Michigan
· 2012
Goldwater
Scholar
Honorable
Mention
· 2011
2 / 3
Vasquez
E
.
,
Thompson
L
. (2020).
Functional
characterization
of
RNA
localization
elements
in
the
3′
UTR
of
CAMK
2
A
.
RNA
,
26(9), 1190–1205.
PROFESSIONAL
ACT IVIT IES
&
CERT IFICAT IONS
Certifications
:
CITI
Program
Human
Research
(
Biomedical
) ·
IACUC
Animal
Handler
Training
·
BSL
-2
and
BSL
-2+
Biosafety
Certified
·
R
Programming
for
Data
Science
(
Johns
Hopkins
/
Coursera
).
Ad
-
hoc
Reviewer
:
Nucleic
Acids
Research
,
Cell
Reports
,
Scientific
Reports
,
PLOS
ONE
.
Professional
Memberships
:
American
Association
for
Cancer
Research
(
AACR
),
American
Society
for
Cell
Biology
(
ASCB
),
RNA
Society
.
Languages
:
English
(
native
),
Spanish
(
fluent
),
French
(
professional
proficiency
).
3 / 3
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